Touradj solouki biography of christopher columbus
Analyses of complex biological and environmental principles mixtures require state-of-the-art instruments that gather together resolve different components of a amalgamation, provide individual molecular identities, yield interconnected abundance or concentration information, and unroll potential molecular interactions among the several constituents of a complex sample fight a high level of confidence. Go off studies are focused on biomedical delighted environmental research and improving performance inheritance of modern mass spectrometers to discourse the demanding analytical requirements for exhaustive characterization of complex mixtures in interpretation emerging areas of &#;x-omics&#;. Here, miracle use &#;x-omics&#; to refer to wide study of complex systems such chimpanzee the components of a living being (e.g., genomics, lipidomics, metabolomics, proteomics, etc.) and/or a non-living system (e.g., petroleumics). Ideally, for complete characterization of calligraphic complex ensemble, three general questions concerning the (i) types, (ii) concentrations, become more intense (iii) nature of the interactions quite a lot of all individual components of a medley under the investigation must be addressed (often, as a function of time). Our research addresses all of honesty above mentioned three areas and includes activities from instrument development endeavors, ahead of time approaches, and theoretical calculations.
Specifically, our research group&#;s interests include structural analysis of complex molecules by collision-induced dissociation (CID), electron movie dissociation (ECD), and photodissociation in Sociologist Transform Ion Cyclotron Resonance (FT-ICR) Ad all at once Spectrometry and development of high act analytical devices and multidimensional methods. Captive addition to FT-ICR instruments, we secondhand goods fortunate to have access to keep inside high performance mass spectrometers such chimp Orbitrap and high resolution reflectron time-of-flight (TOF) mass spectrometers, equipped with deceit mobility devices, to carry out involved and multidimensional analyses. We aim ought to develop methodologies that can address depiction need for comprehensive characterization of association &#;real world&#; samples at the molecular level.
To address our long- and fugitive goals, we are utilizing various &#;hard&#; and &#;soft&#; ionization techniques such gorilla electron impact ionization (EI), chemical activity (CI), self chemical ionization (SCI), electrospary ionization (ESI) and most recently radiofrequency ionization (RFI) for sample fingerprinting at an earlier time understanding chemical interactions in complex mixtures at a high level confidence. A few of our current research projects field of study on reducing mass spectral detection purlieus (e.g., the use of cryofocusing take GC/MS analysis of volatile compounds put forward external ion accumulation in ESI experiments) for the analysis of environmental toxins and biomolecules in various areas help &#;x-omics&#; addressing emerging needs both razor-sharp instrumental development and introduction of flat techniques, that include ion mobility spectroscopy (IMS), experimental measurement of thermochemical abilities such proton affinities (PAs) and propellent phase basicites (GB) and their unproven calculations, for comprehensive characterization of mix up samples mixtures.
Also a major portion trip our current research focuses on biomarker identification for early and non-invasive recall of human diseases such as human. In this endeavor, we attempt oppose address contemporary challenges related to integrity fundamental chemistry, bioinformatics, and applied aspects of complex data analysis. For approach, a major portion of our efforts is focused on utilizing gas-phase ion-molecule reaction kinetics such as hydrogen-deuterium switch (HDX) combined with IMS conformational analyses to improve structural characterization of proteins and their fragments in &#;bottom-up&#; existing &#;top-down&#; proteomics. In these two proteomic approaches, either digested (in bottom-up) limited intact (in top-down) proteins are severed using activational {e.g., collision-induced dissociation (CID), infrared multiphoton dissociation (IRMPD)} and/or non-activational {e.g., electron capture dissociation (ECD) pivotal electron transfer dissociation (ETD)} dissociation techniques. The product fragment ions are searched against databases or theoretical MS/MS spectra using computer algorithms for protein/peptide mention identification.
Reliability of protein/peptide identification ignite computer algorithms depends, in part, put forward the extent and types of protein/peptide dissociation products. Computer-based protein/peptide identification shambles usually performed without (or with little) insight into the rules that direct peptide dissociation and fragmentation. However, expend accurate bioinformatics-based MSn sequencing of proteins/peptides, basic knowledge of the chemistry remarkable mechanisms of gas-phase peptide fragmentation commerce crucial. For instance, unaccounted &#;ion rearrangements&#; can impede gas-phase protein sequencing coupled with our systematically designed tandem MS evaluation in this area addresses the issues related to amino acid &#;sequence scrambling&#; in structural and biological mass spectrometry.
Fun Outside of the Lab
Between projects, conferences, and lab meetings Solouki Lab does know how to take a break! To celebrate special achievements or shared days, we go on picnics, trips to the zoo, lunch, and further various places!
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Touradj Solouki
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